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chromosome-7 bac tile-path array  (Agilent technologies)


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    Structured Review

    Agilent technologies chromosome-7 bac tile-path array
    CGH using the <t>chromosome</t> <t>7</t> (Ch7) BAC tile-path array, Agilent 244A oligonucleotide array platform and chromosome 7 gene resolution oligonucleotide array maps the MDR to a 2.8 Mb region at 7q32 (Panel A). As shown by Affymetrix gene expression analyses, the genes on 7q, particularly those within the MDR are significantly under-expressed in SMZL with 7q deletion than those without the deletion. Panel B shows the genes significantly differentially expressed between SMZL with and without 7q deletion according to P values, while Panel C displays those with further fold change more than 1.25×SD (standard deviation).
    Chromosome 7 Bac Tile Path Array, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bac+array/pmc03441634-109-11-19
    Average 90 stars, based on 1 article reviews
    chromosome-7 bac tile-path array - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "An Integrated Genomic and Expression Analysis of 7q Deletion in Splenic Marginal Zone Lymphoma"

    Article Title: An Integrated Genomic and Expression Analysis of 7q Deletion in Splenic Marginal Zone Lymphoma

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0044997

    CGH using the chromosome 7 (Ch7) BAC tile-path array, Agilent 244A oligonucleotide array platform and chromosome 7 gene resolution oligonucleotide array maps the MDR to a 2.8 Mb region at 7q32 (Panel A). As shown by Affymetrix gene expression analyses, the genes on 7q, particularly those within the MDR are significantly under-expressed in SMZL with 7q deletion than those without the deletion. Panel B shows the genes significantly differentially expressed between SMZL with and without 7q deletion according to P values, while Panel C displays those with further fold change more than 1.25×SD (standard deviation).
    Figure Legend Snippet: CGH using the chromosome 7 (Ch7) BAC tile-path array, Agilent 244A oligonucleotide array platform and chromosome 7 gene resolution oligonucleotide array maps the MDR to a 2.8 Mb region at 7q32 (Panel A). As shown by Affymetrix gene expression analyses, the genes on 7q, particularly those within the MDR are significantly under-expressed in SMZL with 7q deletion than those without the deletion. Panel B shows the genes significantly differentially expressed between SMZL with and without 7q deletion according to P values, while Panel C displays those with further fold change more than 1.25×SD (standard deviation).

    Techniques Used: Expressing, Standard Deviation

    Related Articles

    other:

    Article Title: Inactivation of RUNX3/p46 Promotes Cutaneous T-Cell Lymphoma.
    Article Snippet: Genomic aberrations of 23 SS patients were analyzed by array comparative genomic hybridization (array CGH) using a submegabase resolution BAC array (Erdogan et al., 2006) (6 patients) or a whole genome 400k oligonucleotide array (17 patients; Agilent, Gene Expression Omnibus [GEO] accession no. GPL9777).

    Article Title: Identification of Copy Number Variants Defining Genomic Differences among Major Human Groups
    Article Snippet: Three different genome-wide platforms were used to perform the CNV detection: an in-house developed BAC-based array consisting of ∼32.000 BAC-derived probes from the Human 32 K clone set library (kindly provided by Dr. Joris Veltman), a gene-centered oligonucleotide array of ∼44.000 probes (Human Agilent 44 K; Agilent Technologies Inc, Santa Clara, CA, US) and a genome-wide oligonucleotide array of ∼185.000 probes (Human Agilent 185 K).

    Article Title: An Integrated Genomic and Expression Analysis of 7q Deletion in Splenic Marginal Zone Lymphoma
    Article Snippet: summarised the 7q deletion in SMZL detected by CGH using a chromosome-7 BAC tile-path array (17 cases) and the Agilent aCGH 244A array CGH (10 cases) from our previous studies , .

    Microarray:

    Article Title: CGHpower: exploring sample size calculations for chromosomal copy number experiments
    Article Snippet: .. They will be referred to as Chin et al. [ ], Douglas et al. [ ], Fridlyand et al. [ ], Myllykangas et al. [ ], Nymark et al. [ ], Postma et al. [ ], Smeets et al. [ ] and Wrage et al. [ ] A total of five different array types were used among the data sets: VUmc 30 K spotted oligo [ ] for data sets [ , , ], Agilent Human 1 cDNA Microarray for [ , ], 3 K BAC array [ ] for [ ], 2 K BAC array [ ] for [ ] and 6 K BAC array for [ ]. ..

    Article Title: The Prevalence of Chromosomal Deletions Relating to Developmental Delay and/or Intellectual Disability in Human Euploid Blastocysts
    Article Snippet: Although the Agilent microarray platform has been used for prenatal diagnosis in human clinics , it has not been applied to human PGS. .. Therefore, in this study, we first validated the Agilent DNA microarray platform using pre-known embryo biopsy samples tested by either BAC platform or oligo platform. ..

    BAC Assay:

    Article Title: CGHpower: exploring sample size calculations for chromosomal copy number experiments
    Article Snippet: .. They will be referred to as Chin et al. [ ], Douglas et al. [ ], Fridlyand et al. [ ], Myllykangas et al. [ ], Nymark et al. [ ], Postma et al. [ ], Smeets et al. [ ] and Wrage et al. [ ] A total of five different array types were used among the data sets: VUmc 30 K spotted oligo [ ] for data sets [ , , ], Agilent Human 1 cDNA Microarray for [ , ], 3 K BAC array [ ] for [ ], 2 K BAC array [ ] for [ ] and 6 K BAC array for [ ]. ..

    Article Title: The Prevalence of Chromosomal Deletions Relating to Developmental Delay and/or Intellectual Disability in Human Euploid Blastocysts
    Article Snippet: Although the Agilent microarray platform has been used for prenatal diagnosis in human clinics , it has not been applied to human PGS. .. Therefore, in this study, we first validated the Agilent DNA microarray platform using pre-known embryo biopsy samples tested by either BAC platform or oligo platform. ..

    Article Title: Normalization of array-CGH data: influence of copy number imbalances
    Article Snippet: .. We used eight data sets derived from BAC arrays and from Agilent 244 K oligonucleotide CGH arrays to evaluate normalization methods. ..

    Article Title: Frequency of submicroscopic chromosomal aberrations in pregnancies without increased risk for structural chromosomal aberrations: systematic review and meta-analysis.
    Article Snippet: Publication Array platform/design/resolution AMA1 abnormal cases with AMA indication ANX1 abnormal cases with ANX indication 1 Armengol et al. 2012 BAC array 273 0 (2 SL) 60 0 (1 SL) 2 Lee et al 2012 1-Mb resolution BAC array-based CGH and later 60-K oligonucleotide array-based CGH at 0.5-Mb resolution 1891 9 (1 SL) 983 3 (2 SL) 3 Wapner et al. 2012 Agilent 44K (ca. .. 71% of the cohort) and Affymetrix Genome-Wide Human SNP Array 6.0, containing 1.8 million oligonucleotide probes 1966 92 (25 SL) 0 0 4 Shaffer et al. 2012 BAC-based and oligo-based aCGH platforms: SignatureChipWG V1.0, SignatureChipOS V1.0 2, SignatureChip V4.0 or Signature PrenatalChip V1.0 or V2.0 346 1 95 0 5 Breman et al. 2012 BCM Clinical BAC arrays: BAC V4, V5, V6, OLIGO V6, V7, V8 394 52 0 0 6 Fiorentino et al 2013 BAC array - CytoChip Focus Constitutional (BlueGnome, UK) 1Mb resolution across the genome and 100 kb resolution in 139 significant regions 1118 3 (3 SL) 1675 10 (1 SL) 7 Scott et al. 2013 Agilent ISCA 8×60K array (Agilent, Santa Clara, CA, USA), Agilent ISCA 8×60k design (SUFWprenatal Array), 70 kb resolution backbone with increased probe coverage in targeted regions, CNVs < 400 kb were investigated only if relevant to the referral indication, or in a region of interest targeted by the prenatal array design. .. 393 2 (1 SL) 29 0 8 Konialis et al 2015 BAC array - CytoChip Focus Constitutional (BlueGnome, UK), 1 Mb resolution backbone and a targeted 100 Kb resolution in 139 significant regions 2107 7 (4 SL) Included in AMA category - 9 Papoulidis et al 2015 BAC array – CytoChip Focus Constitutional (BlueGnome, UK) 1- Mb backbone resolution and ~100- kb resolution in 143 significant regions 592 2 (2 SL) 128 0 10 Van Opstal SNP array (Illumina 624 5 0 0 This article is protected by copyright.

    Article Title: Targeting PI3K and RAD51 in Barrett’s Adenocarcinoma: Impact on DNA Damage Checkpoints, Expression Profile and Tumor Growth
    Article Snippet: .. A: BAC cell lines OE33, OE19, and FLO-1 were treated with wortmannin ... Impact of wortmannin on the genome-wide expression profile in FLO-1 cells To further evaluate the impact of wortmannin in the less sensitive FLO-1 cells, we treated cells with wortmannin (20 μM) for 24 h and conducted a genome wide expression profile, using Agilent whole Human Genome arrays (4×44k format). ..

    Derivative Assay:

    Article Title: Normalization of array-CGH data: influence of copy number imbalances
    Article Snippet: .. We used eight data sets derived from BAC arrays and from Agilent 244 K oligonucleotide CGH arrays to evaluate normalization methods. ..

    Genome Wide:

    Article Title: Frequency of submicroscopic chromosomal aberrations in pregnancies without increased risk for structural chromosomal aberrations: systematic review and meta-analysis.
    Article Snippet: Publication Array platform/design/resolution AMA1 abnormal cases with AMA indication ANX1 abnormal cases with ANX indication 1 Armengol et al. 2012 BAC array 273 0 (2 SL) 60 0 (1 SL) 2 Lee et al 2012 1-Mb resolution BAC array-based CGH and later 60-K oligonucleotide array-based CGH at 0.5-Mb resolution 1891 9 (1 SL) 983 3 (2 SL) 3 Wapner et al. 2012 Agilent 44K (ca. .. 71% of the cohort) and Affymetrix Genome-Wide Human SNP Array 6.0, containing 1.8 million oligonucleotide probes 1966 92 (25 SL) 0 0 4 Shaffer et al. 2012 BAC-based and oligo-based aCGH platforms: SignatureChipWG V1.0, SignatureChipOS V1.0 2, SignatureChip V4.0 or Signature PrenatalChip V1.0 or V2.0 346 1 95 0 5 Breman et al. 2012 BCM Clinical BAC arrays: BAC V4, V5, V6, OLIGO V6, V7, V8 394 52 0 0 6 Fiorentino et al 2013 BAC array - CytoChip Focus Constitutional (BlueGnome, UK) 1Mb resolution across the genome and 100 kb resolution in 139 significant regions 1118 3 (3 SL) 1675 10 (1 SL) 7 Scott et al. 2013 Agilent ISCA 8×60K array (Agilent, Santa Clara, CA, USA), Agilent ISCA 8×60k design (SUFWprenatal Array), 70 kb resolution backbone with increased probe coverage in targeted regions, CNVs < 400 kb were investigated only if relevant to the referral indication, or in a region of interest targeted by the prenatal array design. .. 393 2 (1 SL) 29 0 8 Konialis et al 2015 BAC array - CytoChip Focus Constitutional (BlueGnome, UK), 1 Mb resolution backbone and a targeted 100 Kb resolution in 139 significant regions 2107 7 (4 SL) Included in AMA category - 9 Papoulidis et al 2015 BAC array – CytoChip Focus Constitutional (BlueGnome, UK) 1- Mb backbone resolution and ~100- kb resolution in 143 significant regions 592 2 (2 SL) 128 0 10 Van Opstal SNP array (Illumina 624 5 0 0 This article is protected by copyright.

    Article Title: Targeting PI3K and RAD51 in Barrett’s Adenocarcinoma: Impact on DNA Damage Checkpoints, Expression Profile and Tumor Growth
    Article Snippet: .. A: BAC cell lines OE33, OE19, and FLO-1 were treated with wortmannin ... Impact of wortmannin on the genome-wide expression profile in FLO-1 cells To further evaluate the impact of wortmannin in the less sensitive FLO-1 cells, we treated cells with wortmannin (20 μM) for 24 h and conducted a genome wide expression profile, using Agilent whole Human Genome arrays (4×44k format). ..

    Expressing:

    Article Title: Targeting PI3K and RAD51 in Barrett’s Adenocarcinoma: Impact on DNA Damage Checkpoints, Expression Profile and Tumor Growth
    Article Snippet: .. A: BAC cell lines OE33, OE19, and FLO-1 were treated with wortmannin ... Impact of wortmannin on the genome-wide expression profile in FLO-1 cells To further evaluate the impact of wortmannin in the less sensitive FLO-1 cells, we treated cells with wortmannin (20 μM) for 24 h and conducted a genome wide expression profile, using Agilent whole Human Genome arrays (4×44k format). ..



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    Image Search Results


    CGH using the chromosome 7 (Ch7) BAC tile-path array, Agilent 244A oligonucleotide array platform and chromosome 7 gene resolution oligonucleotide array maps the MDR to a 2.8 Mb region at 7q32 (Panel A). As shown by Affymetrix gene expression analyses, the genes on 7q, particularly those within the MDR are significantly under-expressed in SMZL with 7q deletion than those without the deletion. Panel B shows the genes significantly differentially expressed between SMZL with and without 7q deletion according to P values, while Panel C displays those with further fold change more than 1.25×SD (standard deviation).

    Journal: PLoS ONE

    Article Title: An Integrated Genomic and Expression Analysis of 7q Deletion in Splenic Marginal Zone Lymphoma

    doi: 10.1371/journal.pone.0044997

    Figure Lengend Snippet: CGH using the chromosome 7 (Ch7) BAC tile-path array, Agilent 244A oligonucleotide array platform and chromosome 7 gene resolution oligonucleotide array maps the MDR to a 2.8 Mb region at 7q32 (Panel A). As shown by Affymetrix gene expression analyses, the genes on 7q, particularly those within the MDR are significantly under-expressed in SMZL with 7q deletion than those without the deletion. Panel B shows the genes significantly differentially expressed between SMZL with and without 7q deletion according to P values, while Panel C displays those with further fold change more than 1.25×SD (standard deviation).

    Article Snippet: summarised the 7q deletion in SMZL detected by CGH using a chromosome-7 BAC tile-path array (17 cases) and the Agilent aCGH 244A array CGH (10 cases) from our previous studies , .

    Techniques: Expressing, Standard Deviation

    Clinical cycle of comprehensive preimplantation genetic testing. The process starts with family genetic testing in order to establish normal and disease-associated family haplotypes. Next, oocyte stimulation, retrieval, and fertilization are performed. Embryos surviving until day 5 undergo laser-assisted hatching and biopsy. Biopsy material consisting of a few trophectoderm cells is amplified using one of the WGA techniques. WGA material is further used to assess the embryonic genome, including haplotype, causative variant, aneuploidy, and CNV analyses. The disease-free embryo is subjected to embryo transfer to the uterine cavity. The whole cycle can be repeated within 24 h, with a day-6 embryo being transferred. If the genetic testing takes longer, then embryos are vitrified and thawed before being transferred. PGT—preimplantation genetic testing, WGA—whole-genome amplification, IVF—in vitro fertilization, MPS—massively parallel sequencing, CNV—copy number variation.

    Journal: International Journal of Molecular Sciences

    Article Title: Whole Genome Amplification in Preimplantation Genetic Testing in the Era of Massively Parallel Sequencing

    doi: 10.3390/ijms23094819

    Figure Lengend Snippet: Clinical cycle of comprehensive preimplantation genetic testing. The process starts with family genetic testing in order to establish normal and disease-associated family haplotypes. Next, oocyte stimulation, retrieval, and fertilization are performed. Embryos surviving until day 5 undergo laser-assisted hatching and biopsy. Biopsy material consisting of a few trophectoderm cells is amplified using one of the WGA techniques. WGA material is further used to assess the embryonic genome, including haplotype, causative variant, aneuploidy, and CNV analyses. The disease-free embryo is subjected to embryo transfer to the uterine cavity. The whole cycle can be repeated within 24 h, with a day-6 embryo being transferred. If the genetic testing takes longer, then embryos are vitrified and thawed before being transferred. PGT—preimplantation genetic testing, WGA—whole-genome amplification, IVF—in vitro fertilization, MPS—massively parallel sequencing, CNV—copy number variation.

    Article Snippet: PGT performed on trophectoderm biopsies using PicoPLEX in combination with BlueGnome’s BAC arrays (later Illumina, now discontinued) and subsequently MPS began a major revolution in the availability of human PGT and forever changed the face of clinical in vitro fertilization [ , , ].

    Techniques: Amplification, Variant Assay, Whole Genome Amplification, In Vitro, Sequencing